I have ~2,000 whole-genome-sequencing bams that I need to move through an alignment/deduping/recalibration pipeline. I'll be using bwa-mem for the initial alignment, and I'm wondering if you would still recommend carrying out the local realignment step (downstream) for these bams, given bwa-mem does some local/gapped alignment on its own. This is an important question for our group because the local realignment (using RealignerTargetCreator & IndelRealigner) for these bams will take a significant amount of time / computational resources. If I want to call short indels using UnifiedGenotyper (rather than HC, due to number of samples), does bwa-mem for primary alignment reduce the need for local realignment downstream? Any feedback/thoughts would be much appreciated - thanks!