I am writing a pipeline to analyse exome sequencing data from families and would like some advice on using the VariantAnnotator walker. It seems I need to use VariantAnnotator prior to VariantRecalibrator as the latter relies on annotations generated by VariantAnnotator. Is this correct? Also, I would like to annotate my VCF using SNPeff and SNPsift. Can you suggest the best order in which to perform these steps. For example, my plan at the moment is to run steps in the following order: HaplotypeCaller, VariantAnnotator, VariantRecalibrator, ApplyRecalibration, PhaseByTransmission, ReadBackedPhasing, SNPeff, SNPsift.
Any help would be much appreciated,