I just run HyplotypeCaller on a dataset. For the same dataset, I have run through Unified genotyper and then directly subjected the raw vcf from UG to VQSR step without the help of VariantAnnotator before and get through VQSR without any problem. However, when I try to subject the raw callset derived from HyplotypeCaller directly to VQSR step, the VQSR module complained about it and error message is below:
So after HyplotypeCaller, the derived vcf file needs to run though VariantAnnotator? Since Unified genotyper derived callset does not need the help of VariantAnnotator (all annotations needed for VQSR are included after UG), it seems not the case for HyplotypeCaller? I can run through VariantAnnotator for HyplotypeCaller derived vcf file, just want to make sure if my understanding is correct?
Thanks and best