Hi,
I have downloaded newest version of GATK (version 2.4-3) this week and tried to perform local realignment for my targeted sequencing data. Reference genome, SNP and indel data files were downloaded from resource bundle. However, I encountered two issues when I was doing the realignment.
First, in the step of RealignerTargetCreator. With the same command line, if I run it under version 2.4-3, I got an error message "MESSAGE: -49" (no other detail information provided); if I run it under an older version 2.3-9, it ran very well with no errors.
Second, in the step of IndelRealigner. I got error message "MESSAGE: Bad input: We encountered a non-standard non-IUPAC base in the provided reference: '13'". However, reference genome was downloaded from the bundle. I am not sure how to fix this issue.
I hope someone can help me with these issues. Let me know if more info is needed.
Thanks!
