UG calling a SNP as heterozygous when it (most likely) isn't
Posted in Ask the GATK team | Last updated on

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The title kind of explains the situation, but basically I've got a SNP that shows up in IGV that I would call homozygous that the Unified Genotyper has labeled as heterozygous. The total read depth is 35, 32 of which were called as a SNP (A-->T), 2 were called the reference base (A), and one read contained a G. I went through your article describing why a SNP visible in IGV might not get called, and none of those five questions explained this situation. I didn't alter the --hets option at all either. Any help you might be able to offer would be greatly appreciated.

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