Will it be different if I seperate snp and indel to run VQSR
Posted in Ask the team | Last updated on 2013-01-07 20:02:39

Comments (1)

Hi All, I have used GATK UnifiedGenotyper to generate a raw.vcf file. Now I want to use GATK VQSR to get a more accurate result ,and I follow this protocol:

snp.model <- BuildErrorModelWithVQSR(raw.vcf, SNP);
indel.model <- BuildErrorModelWithVQSR(raw.vcf, INDEL);
recalibratedSNPs.rawIndels.vcf <- ApplyRecalibration(raw.vcf, snp.model, SNP);
analysisReady.vcf <- ApplyRecalibration(recalibratedSNPs.rawIndels.vcf, indel.model, INDEL);

I wanna know will the it be better if I seperate the SNP and INDEL to run VQSR, like this:

SNP.raw.vcf , INDEL.raw.vcf <- SeperateSNPINDEL(raw.vcf);
snp.model <- BuildErrorModelWithVQSR(SNP.raw.vcf, SNP);
indel.model <- BuildErrorModelWithVQSR( INDEL.raw.vcf, INDEL);
SNP_analysisReady.vcf <- ApplyRecalibration(SNP.raw.vcf, snp.model, SNP);
INDEL_analysisReady.vcf <- ApplyRecalibration(INDEL.raw.vcf, INDEL.model, SNP);

Thanks a lot !

Return to top Comment on this article in the forum