Cancer Program Publication

A Mechanism of Cyclin D1 Action Encoded in the Patterns of Gene Expression in Human Cancer
ProjectTranscriptional Regulation
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Categories
Bioinformatics & Computational Biology
AbstractHere we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a previously unappreciated mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPβ/Nf-Il6, participates in the consequences of cyclin D1 overexpression. Functional analyses confirmed the involvement of C/EBPβ in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.
AuthorsJustin Lamb, Sridhar Ramaswamy, Heide L. Ford, Bernardo Contreras, Robert V. Martinez, Frances S. Kittrell, Cynthia A. Zahnow, Nick Patterson, Todd R. Golub and Mark E. Ewen
Publication Date08/08/2003
Contact emails mark_ewen@dfci.harvard.edu
justin@genome.wi.mit.edu
CitationCell 114: 323-334 2003
 
Supplemental Information
URLs
NameURL
full texthttp://download.cell.com/pdfs/0092-8674/PIIS0092867403005701.pdf
Preview in Cellhttp://download.cell.com/pdfs/0092-8674/PIIS0092867403006019.pdf
Nature News and Viewshttp://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v424/n6951/full/424898a_fs.html&content_filetype=PDF
Nature Reviews in Cancer Highlightshttp://www.nature.com/cgi-taf/DynaPage.taf?file=/nrc/journal/v3/n9/full/nrc1175_fs.html&filetype=pdf
Nature Genetics Research Noteshttp://www.nature.com/cgi-taf/DynaPage.taf?file=/ng/journal/v35/n1/full/ng0903-19.html&filetype=PDF
Files
DescriptionFile
Supplementary Information 1 (cyclin D1 nearest neighbors)SI1.txt
Supplementary Information 2 (GCM KSS)SI2.txt
Supplementary Information 3 (prostate KSS)SI3.txt
Supplementary Information 4 (lung KSS)SI4.txt
Supplementary Information 5 (brain KSS)SI5.txt
Supplementary Information 6 (NCI60 KSS)SI6.txt
Supplementary Information 7 (chromosome coordinates)SI7.pdf
Supplementary Information 8 (competitor oligonucleotides)SI8.pdf
Supplementary Information 9 (additional panels for Figure 6D)SI9.pdf
Supplementary Information 10 (additional panels for Figure 6E)SI10.pdf
raw gene expression dataraw_data.res