Figure 3: NKX2-1 RNAi leads to reduced anchorage-independent growth and viability of NCI-H2009 cells but not A549 cells. a) Anti-NKX2-1 and anti-vinculin control immunoblots of lysates from NCI-H2009 and A549 cells expressing shRNA against NKX2-1 (shNKX2-1a and b) or GFP (shGFP) as control. b) Soft agar colony formation by NCI-H2009 cells is shown relative to the shGFP control as a mean percentage (+/- standard deviation in triplicate samples; p = 5.8 x 10-6 when comparing shGFP to shNKX2-1a and p = 5.1 x 10-4 when comparing shGFP to shNKX2-1b). c) Colony formation assays for A549 cells as in part b (p>0.5). d) Anti-MBIP and anti-vinculin immunoblots of lysates from shRNA-expressing NCI-H2009 cells. e) Colony formation of NCI-H2009 cells relative to that of control shGFP cells (p = 0.0344).