Symbols indicating the kind of ground truth available:
Counts
Foreground and background
Outlines of individual objects
Biological label (positive and negative images, or a dose response curve)
SBS Vitra CNT
 |
 |
| positive | negative |
Biological application
This 96-well plate has images of cytoplasm to nucleus translocation of the transcription factor NFκB in MCF7 (human breast adenocarcinoma cell line) and A549 (human alveolar basal epithelial) cells in response to TNFα concentration.
Images
Images are at 10x objective magnification. The plate was acquired at Vitra Bioscience on the CellCard reader. For each well there is one field with two images: a nuclear counterstain (DAPI) image and a signal stain (FITC) image. Image size is 1360 x 1024 pixels. Images are in 8-bit BMP format.
Ground truth
File name structure is <channel>-<well-number>-<row>-<column>-<field>.BMP
The plate map is a standard 96-well plate, containing 12 concentration points in columns and 4 replica rows for each cell type.
The following files encodes this plate map in the format expected by CellProfiler's LoadText module:
Published results using this image set
Z'-factor:
| 0.92 (Texture Variance in Cells) | Carpenter et al., Genome Biology, 2006 | Figure 4C, pages 8-9 |
V-factor:
| 0.88 (Thresholded Cytoplasm/Nuclei Intensity) | Logan and Carpenter, Journal of Biomolecular Screening 2010 | Figure 2B |
Files to reproduce results in Logan and Carpenter, Journal of Biomolecular Screening 2010:
Vitra_files.zip (Includes CellProfiler pipelines and metadata.)For more information
These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.
Recommended citation
"We used the SBS Vitra CNT image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."
Copyright
The SBS Vitra CNT images are licensed under a Creative Commons Attribution 3.0 Unported License by Ilya Ravkin.