Symbols indicating the kind of ground truth available:
Counts
Foreground and background
Outlines of individual objects
Biological label (positive and negative images, or a dose response curve)
SBS Roche Transfluor
 |
 |
| positive | negative |
Description of the biological application
This image set is of a Transfluor assay where an orphan GPCR is stably integrated into the b-arrestin GFP expressing U2OS cell line. After one hour incubation with a compound the cells were fixed with (formaldehyde).
Images
The plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. File format is 8-bit TIFF with one image for green channel (GFP) and one image for blue channel (DNA). Image size is 512 x 512 pixels.
Ground truth
The images constitute one row of a 384 well plate. The dose curve consists of 11 dose points and one control. Each concentration is duplicated in adjacent wells. Each well has three fields. File name structure: <prefix>_<row><column><field><channel>.TIF
The following file encodes this plate map in the format expected by CellProfiler's LoadText module:
Published results using this image set
Z'-factor:
| 0.43 (Integ. Intensity on edge of Green Speckles) | Carpenter et al., Genome Biology, 2006 | Figure 3 |
V-factor:
| 0.67 (Radial Distribution CV of intensity) | Logan and Carpenter, Journal of Biomolecular Screening 2010 | Figure 3 |
Files to reproduce results in Logan and Carpenter, Journal of Biomolecular Screening 2010:
Roche_files.zip (Includes CellProfiler pipelines and metadata.)For more information
These images were originally gathered for Ilya Ravkin's Invitation to Participate in the Comparison of Image Analysis Algorithms for Intracellular Screening.
Recommended citation
"We used the SBS Roche Transfluor image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."
Copyright
The SBS Roche Transflour images are licensed under a Creative Commons Attribution 3.0 Unported License by Ilya Ravkin.