Broad Institute

Broad Bioimage Benchmark Collection

Annotated biological image sets for testing and validation

Image sets

Identification and segmentation
Accession Description Mode Fields Ground truth
BBBC001 Human HT29 colon-cancer cells Fluorescent 6 C
BBBC002 Drosophila Kc167 cells Fluorescent 50 C
BBBC003 Mouse embryos DIC 15 C F
BBBC004 Synthetic cells “Fluorescent” 100 C F
BBBC005 Synthetic cells “Fluorescent” 19,200 C F
BBBC006 Human U2OS cells (out of focus) Fluorescent 52,224 F O
BBBC007 Drosophila Kc167 cells Fluorescent 16 O
BBBC008 Human HT29 colon-cancer cells Fluorescent 12 F
BBBC009 Human red blood cells DIC 5 O
BBBC010 C. elegans live/dead assay (uneven illumination) Bright field and fluorescent 384 F O B
BBBC018 Human HT29 colon-cancer cells (diverse phenotypes) Fluorescent 55 O
BBBC019 Collective cell migration DIC 126 F
BBBC020 Murine bone-marrow derived macrophages Fluorescent 20 O

Phenotype classification
Accession Description Mode Fields Ground truth
BBBC010 C. elegans live/dead assay Bright field and fluorescent 384 F O B
BBBC011 C. elegans metabolism assay Bright field and fluorescent 50 B
BBBC012 C. elegans infection marker Bright field and fluorescent 120 B
BBBC013 Human U2OS cells cytoplasm–nucleus translocation Fluorescent 96 B
BBBC014 Human U2OS cells cytoplasm–nucleus translocation Fluorescent 96 B
BBBC015 Human U2OS cells transfluor Fluorescent 144 B
BBBC016 Human U2OS cells transfluor Fluorescent 144 B

Complete high-throughput experiments without ground truth
Accession Description Mode Fields Ground truth
BBBC017 Human HT29 colon-cancer cells shRNAi screen Fluorescent 64,512