Genome Diversity During Transmission: Mosquito to Bird

Justification/Summary

How do mosquitoes and birds differentially affect West Nile virus population dynamics?

Different components of the WNV transmission cycle are thought to have distinct affects on virus populations.  It was recently shown that intra-host genetic diversity was greater in mosquitoes than birds, and that the evolutionary mechanism for this was a relative relaxation of purifying selection in mosquitoes.  However, the potential bottlenecks that occur as a result of either mosquito or bird infection, and whether they are neutral or selectively determined (such that specific variants are selected at transmission), have not been systematically examined.  Therefore intra-host population dynamics and migration patterns will be examined in two representative hosts: Culex mosquito vectors, day-old chickens, and alternating mosquito and bird infections.

People

Principal External Collaborators

Gregory D. Ebel - Dept. of Pathology, University of New Mexico School of Medicine, Albuquerque, NM

Laura D. Kramer - Wadsworth Center Arbovirus Lab, New York State Dept. of Health, Slingerlands, NY

Funding

Funding for this sequencing project was provided by the National Institute of Allergy and Infectious Diseases.

Cohort Description

CX_Intra - Virus harvested from the midgut, hemolymph or salivary secretions of culex mosquito vectors fed with diverse WNV populations.

WN-H_CK-BL - Virus harvested from the blood of chicks infected with a homogeneous WNV population

WN-MX_CK-BL - Virus harvested from the blood of chicks infected with a heterogeneous WNV population

WN-H_CK-KD - Virus harvested from the kidneys of chicks infected with a homogeneous WNV population

WN-MX_CK-KD - Virus harvested from the kidneys of chicks infected with a heterogeneous WNV population

WN-H_CK-BR - Virus harvested from the brains of chicks infected with a homogeneous WNV population

WN-MX_CK-BR -  Virus harvested from the brains of chicks infected with a heterogeneous WNV population

WN-H_CK-SK - Virus harvested from the skin of chicks infected with a homogeneous WNV population

WN-MX_CK-SK -  Virus harvested from the skin of chicks infected with a heterogeneous WNV population