Protocols

Please click on the links below to download the latest versions of these documents:

John-etal_2010-EM.pdf

Analysis of High-Throughput Sequencing and Annotation Strategies for Phage Genomes (published at PLoS ONE)

243 KB

journal.pone.0009083.pdf

Preparation of Cyanophage DNA from a Cleared Lysate

651 KB

phageProtocols_ChisholmLab_LysateDNA-Prep_release20090521-r1.pdf

Linker Amplified Shotgun Library Protocol

83 KB

phageProtocols_Broad_LASL_release20070919.pdf

Whole Genome Shotgun Library Protocol

290 KB

emi_2791.pdf

Sample files for assembly, annotation and file format tutorial

2.1 MB

phageProtocols_Broad_WGS_release20070907.pdf

Modified LASL protocol optimized for low template quantities and pyrosequencing

219 KB

phageTutorial_files.zip

Laboratory procedures to generate viral metagenomes (published at Nature Protocols)

294 KB

phageProtocols_SullivanLab_ModifiedLinkerAmplification-TMPL_Ver.4-1.pdf

Frequently Asked Questions about the Marine Phage, Virus and Virome Sequencing Project

134 KB

phageProtocols_Thurber_etal_Nature2009.pdf

584 KB

phage_gbmfSeqInitiativeFAQs_release20090512.pdf

140 KB

phageTutorial_Audio.zip

Audio recording (mp3); tutorial starts at approx. 8'30"

29 MB