Protocols

Please click on the links below to download the latest versions of these documents:

John-etal_2010-EM.pdf Analysis of High-Throughput Sequencing and Annotation Strategies for Phage Genomes (published at PLoS ONE)	243 KB
journal.pone.0009083.pdf Preparation of Cyanophage DNA from a Cleared Lysate	651 KB
phageProtocols_ChisholmLab_LysateDNA-Prep_release20090521-r1.pdf Linker Amplified Shotgun Library Protocol	83 KB
phageProtocols_Broad_LASL_release20070919.pdf Whole Genome Shotgun Library Protocol	290 KB
emi_2791.pdf Sample files for assembly, annotation and file format tutorial	2.1 MB
phageProtocols_Broad_WGS_release20070907.pdf Modified LASL protocol optimized for low template quantities and pyrosequencing	219 KB
phageTutorial_files.zip Laboratory procedures to generate viral metagenomes (published at Nature Protocols)	294 KB
phageProtocols_SullivanLab_ModifiedLinkerAmplification-TMPL_Ver.4-1.pdf Frequently Asked Questions about the Marine Phage, Virus and Virome Sequencing Project	134 KB
phageProtocols_Thurber_etal_Nature2009.pdf	584 KB
phage_gbmfSeqInitiativeFAQs_release20090512.pdf	140 KB

phageTutorial_Audio.zip

Audio recording (mp3); tutorial starts at approx. 8'30"

29 MB