Mycoplasma mobile Assembly

Methodology Overview

The Mycoplasma mobile genome was sequenced using the Whole Genome Shotgun methodology, whereby:
  1. High-molecular weight genomic DNA is isolated from Mycoplasma mobile 163K (ATCC 43663) and subsequently sheared into smaller-sized fragments (~2 kb, ~4 kb, ~6 kb, ~8 kb, and ~10 kb)
  2. DNA fragments are size-fractionated, ligated into the appropriate vector and subsequently amplified in E. coli cells.
  3. The two ends of each cloned DNA fragment are sequenced, creating paired reads.
  4. The assembly process uses the paired reads to identify contiguous stretches of sequence (contigs).
  5. Contigs are ordered and linked together into larger supercontigs by using paired reads lying in different contigs.
  6. Standard finishing methods are applied to resolve low-quality regions (sequence ambiguities) and to obtain additional sequence from specific genome regions that are missing from the original assembly.

Assembly Data

Final Assembly

Sequencing Facts

  • Greater than 10X sequencing coverage of the genome
  • Circular genome with a size of 777,079 bp
  • G+C content 24.95%

Genomic Libraries: Details

We end sequenced plasmid libraries.

Library Type Vector Average Insert Size (Kb) # Attempted Reads # Reads in Assembly (final version)
1PlasmidpOT2.015,8524,376
2PlasmidpOT4.026,6947,152
3PlasmidpJAN6.05,6641,628
4PlasmidpJAN8.05,6641,617
5PlasmidpJAN10.05,6641,603