The Uncinocarpus reesii sequencing project is part of the Broad Institute Fungal Genome Initiative. Its goal is to release an annotated assembly with 4X genome sequence coverage for Uncinocarpus reesii strain 1704. Dr. Taylor's lab at University of Berkley provided the genomic DNA for the sequencing project.
Our specific aims are as follows:
- Generate and assemble sequence reads yielding 4X coverage of the U. reesii genome through whole genome shotgun sequencing.
- Perform automated annotation of the sequence assembly.
- Distribute the sequence assembly and results of our annotation and analysis through a freely accessible, public web server at the Broad and by deposition of the sequence assembly in GenBank.
We produced whole genome shotgun sequence from two plasmid libraries (4kb and 10kb inserts) and a Fosmid library. The resulting 5X assembly was made public in September, 2005, and the results of automated genome annotation will be made public in future releases.
Questions about the project should be directed to annotation-webmaster(at)broad.mit.edu.
What is Unicinocarpus reesii?
Unicinocarpus reesii is morphologically very similar to Coccidioides species and sequence analysis indicates that it is one of the closest known relative of Coccidioides. The sequence divergence of the 18S ribosomal gene between C. immitis and U. reesii is approximately 0.7%, reflecting approximately 20-30 million years evolutionary distance (Bowman, White et al. 1996).
Coccidioides species cause serious and sometimes fatal disease (coccidiomycosis) in otherwise healthy people. The U.S. government regulates both C. posadasii and C. immitis under the Select Agent Program as potential bioterrorist threats. For this reason, Coccidioides is an active target of molecular genetic and clinical investigation. The genome sequences of both C. posadasii and C. immitis are now available. However, unlike these Coccidioides species, U. reesii is nonpathogenic. Having the sequence from these three closely related fungi will allow a comparative approach to the study of pathogenesis in Coccidioides.
Bowman BH, White TJ and Taylor JW. 1996 Human Pathogenic Fungi and their close Naopathogenic relatives. Molecular Phylogenetics and Evolution 6(1): 89-96