Assembly Description

Methodology Overview

The Pseudomonas aeruginosa genomes were sequenced using the Whole Genome Shotgun methodology, whereby:
  1. Pseudomonas aeruginosa DNA is randomly shattered into small fragments.
  2. Narrow range fragments around 4 Kb, 10 Kb & 40 Kb are inserted into vectors to create 4 Kb & 10 Kb plasmids and 40 Kb Fosmids.
  3. The two ends of the fragment are sequenced, creating paired reads
  4. The assembly process uses the paired reads to identify contiguous stretches of sequence (contigs)
  5. Contigs are ordered and linked together into larger supercontigs by using paired reads lying in different contigs
assembly diagram

Assembly Data


SupercontigsContigsCoverageContig NC50Supercontig NC50
P. aeruginosa 21921 82 (6,146,998 bp)9.5X(8X,Q>20)X174.7 Kb6.9 Mb
P. aeruginosa c37191124 (6,146,998 bp)9.5X(8X,Q>20)X98.8 Kb6.2 Mb

Supercontig/Contig Numbering

  • Supercontig and contig numbers are preceded by the version of the assembly. For example:
    • Contig 1.2 - refers to contig number 2 within assembly 1.
    • Supercontig 1.1 - refers to supercontig number 1 within assembly 1.
  • Supercontigs are numbered in order of decreasing length. See the Assembly Structure page for a list of all supercontigs with their lengths and contained contigs.
  • Contigs within supercontigs are ordered positionally within a supercontig. See the Assembly Structure page for a list of all supercontigs with their lengths and contained contigs.

    There is no correspondence between contig or supercontigs numbers in different assemblies.

Library Clones

Pseudomonas aeruginosa 2192

Type Clone ends generated Clone ends mapped to assembly
Plasmid 82,172 71,311
Fosmid Library pseudomonas_aeruginosa_2192 G775F1/F2 13,823 11,325
Totals 95,995 82,636

Pseudomonas aeruginosa c3719

Type Clone ends generated Clone ends mapped to assembly
Plasmid 81,216 76,358
Fosmid Library pseudomonas_aeruginosa_c3719 WIBR-G782-F1 11,136 10,312
Totals 92,352 86,670