Descriptions

Outline

Click the species name for a description of the organism

P. brasiliensis Pb01

Pb01 is a clinical isolate from an acute form of PCM in an adult male. So far, this is the most thoroughly studied isolate at the molecular level, as deduced from an extensive analysis of its transcriptome that yielded ESTs covering about 80% of the estimated genome of P. brasiliensis (Felipe et al. 2005). The nuclear genome size was estimated by PFGE at around 30 Mb (Montoya et al. 1997).

P. brasiliensis Pb03

Pb03 is one of the better-studied isolates of PS2. It has been used in molecular, phylogenetic, and virulence studies. Transcriptional regulation seems to vary in Pb03 when compared with Pb18, as suggested by the down regulation of PbGP43 due to growth temperature increase (Carvalho et al. 2005). Accordingly, the heat shock genes PbLON and PbMDJ1 are up regulated more slowly in Pb03 than in Pb18 after a temperature shift from 36oC to 42oC. In Pb18, but not in Pb03, PbMDJ1 seems to be preferentially expressed in yeast, as suggested by real time RT-PCR analysis of the genes during mycelium-to-yeast-to mycelium transition. Pb03 has four chromosomal bands separated by PFGE. The size of each chromosome is 9.5, 6.4, 5.3 and 3.0 Mb, respectively. This gives a haploid genome of about 30 Mb.

P. brasiliensis Pb18

Pb18 is the representative of the major phylogenetic group S1 and has been extensively used in the literature due to its proven virulence in mice when inoculated by the intraperitoneal (i.p.), intratracheal (i.t.) and intravenous (i.v.) routes (Calich et al. 1998). Chromosomes of Pb18 can be separated by pulsed field gel electrophoresis (PFGE) in 4-5 distinct bands. The haploid genome size is approximately 30 Mb (Almeida et al. 2006). Analysis of its transcriptome yielded ESTs covering about 40 to 50 % of the estimated genome of P. brasiliensis (Felipe et al. 2005). A microarray based on these cDNAs was already developed and is available to the community (Nunes et al., 2005).