Mycobacterium tuberculosis Diversity Database
Different M. tuberculosis (TB) strains have varying virulence, immunogenicity, and drug resistance. However, currently sequenced TB genomes are thought to be responsible for only a minority of global TB burden. This whole-genome sequencing project proposes to exploit recently revealed TB global population structure to target 31 rationally selected TB strains, to be sequenced as the representatives of the TB global diversity.
Sequencing the genomes of many TB strains is an important step towards understanding the molecular basis of the differences in virulence, immunogenicity, and drug resistance. Multiple TB genome sequences have become available; however, these sequences represent only two of the six main lineages of TB, which are thought to be responsible for only a minority of global TB burden. Therefore, much of the genome sequence diversity of potentially important TB strains remains to be characterized.
Recent advances in comparative genomics have revealed the global population structure of TB. This project proposes to exploit this structure to rationally select 31 TB strains, representative of the global diversity of TB, for whole-genome sequencing. A sequencing coverage of 8X will be used for strains from lineages for which no genome has been sequenced (9 strains), followed by 3X coverage to sequence additional representatives to explore within lineage diversity (22 strains).