Cryptococcus gattii Database
Cryptococcus species are human pathogens which have been diverging over the past 40 million years into three distinct varieties and four serotypes: the predominantly opportunistic pathogens C. neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) and the primary pathogen C. gattii (serotypes B and C). C. neoformans is the leading cause of infectious meningitis with high incidence in AIDS patients. C. gattii is notable in causing infections of immunocompetent individuals. C. gattii isolate R265 caused an outbreak on Vancouver Island in Canada that involved more than 50 infections in otherwise healthy human patients, with at least four fatalities (Fyfe, Black et al. 2002). The outbreak isolate R265 has a VGII molecular genotype by PCR fingerprinting, which differs from the VGI molecular genotype of strain WM276 (J. Kronstad, personal communication). This database includes the R265 genome sequenced by the Broad Institute and the WM276 genome contributed by the University of British Columbia.
This sequence project is part of the Broad Fungal Genome Initiative. Its goal is to release a genome assembly with 4X sequence coverage for Cryptococcus gattii isolate R265, the causal agent of an outbreak on Vancouver Island in Canada. Drs. Joseph Heitman and James Fraser at Duke University Medical Center provided the genomic DNA for the sequencing project.Our specific aims are as follows:
- Generate and assemble sequence reads yielding 4X coverage of the Cryptococcus gattii isolate R265 genome through whole genome shotgun sequencing.
- Perform automated annotation of the sequence assembly.
- Distribute the sequence assembly and results of our annotation and analysis through a freely accessible, public web server at the Broad and by deposition of the sequence assembly in GenBank.