Blastomyces dermatitidis Database
Blastomyces dermatitidis is the causative agent of blastomycosis, a geographically widespread systemic mycosis of humans and other mammals. Although blastomycosis occurs worldwide, it is most common in North America and is endemic to the Ohio and Mississippi River Valley regions. After mild infection, blastomycosis can undergo a prolonged latent period and reactivate in individuals with compromised immune systems, as is the case in AIDS patients. Untreated acute blastomycosis frequently progresses to severe pulmonary disease, with potential for dissemination to other organs, skin and bone.
We have sequenced four strains of Blastomyces dermatitidis. The SLH-14081 strain is a highly virulent, clinical isolate that can cause disease in immunocompetent people. Comparative analysis between the SLH-14081 strain and an avirulent strain, ER-3, provides the opportunity to identify genomic attributes unique to the virulent SLH-14081 strain and therefore, important in blastomycosis. We have also sequenced ATCC18188, a representative MAT 'a' isolate, and ATCC26199 (in collaboration with the Washington University Genome Sequencing Center), a commonly used laboratory isolate. This project aims to examine diversity between the four Blastomyces dermatitidis strains and determine the common and unique features among the larger group of dimorphic fungal pathogens. The comparative Blastomyces dermatitidis project is funded by NIAID.
The Blastomyces dermatitidis sequencing project is part of the Broad Institute Fungal Genome Initiative. Its goal is to release an annotated assemblies for Blastomyces dermatitidis strains ER-3, SLH-14081, ATCC18188, and ATCC26199.
For the Blastomyces dermatitidis SLH-14081 and ER-3 strains, we produced whole genome shotgun sequence from epiFOS, pJAN and pOT libraries. The resulting 8.45X and 9.43X assemblies were made public in December 2008, and the results of automated genome annotation were completed in April 2009.
For the Blastomyces dermatitidis ATCC18188, we produced whole genome shotgun sequence from small insert libraries (fragment and 1.5kb) using 454 technology and from Fosmids using Sanger technology. The resulting 21X assembly was made public in March 2010, and the results of automated genome annotation will be released later this year.
Questions about the project should be directed to annotation webmaster.
The primary collaborators for this project are:
- Bruce Klein, University of Wisconsin, Madison
- Thomas Sullivan, University of Wisconsin, Madison
- Joseph Heitman, Duke University Medical Center
- Wenjun Li, Duke University Medical Center
- Klein BS, Tebbets B Dimorphism and virulence in fungi. Curr Opin Microbiol. 2007 Aug;10(4):314-9. Epub 2007 Aug 23. Review
- McCullough MJ et al. Molecular epidemiology of Blastomyces dermatitidis. Clin Infect Dis. 2000, 30(2):328-35
- Hogan LH, Klein BS, Levitz SM. Virulence factors of medically important fungi. Clin Microbiol Rev. 1996 Oct;9(4):469-88. Review.